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Analyse et comparaison des niveaux de contamination en Polychlorobiphényles et pesticides organochlorés dans les foies de juvéniles de soles provenant de nourriceries du Golfe de Gascogne, de la Baie de Seine et de la Somme ArchiMer
Jegaden, Marine.
Ce rapport de stage (2 mois et demi) traite du dosage de contaminants organiques, les Polychlorobiphényles (PCB) et les pesticides organochlorés chez les juvéniles de soles, dans différentes zones côtières de nourriceries sur le littoral français en Manche Est et dans le Golfe de Gascogne. Ces composés, hydrophobes, persistants et potentiellement bioaccumulables, se trouvent concentrés dans les tissus des poissons et en particulier dans le foie, riche en lipides. Les 16 congénères de PCB et les pesticides organochlorés sont dosés selon un protocole comprenant trois étapes : l'extraction, la purification par chromatographie d'adsorption pour séparer les composés, puis la quantification par chromatographie en phase gazeuse munie d'un détecteur à capture...
Tipo: Text Palavras-chave: Soles; Nourriceries; PCB; Pesticides organochlorés; Bioaccumulation; Métabolisation; Réseau National d'Observation.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00444/55522/57110.pdf
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In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes ArchiMer
Lambert, Christophe; Soudant, Philippe; Jegaden, Marine; Delaporte, Maryse; Labreuche, Yannick; Moal, Jeanne; Samain, Jean-francois.
Bivalve hemocyte competence has been measured by quantifying functional characteristics, including reactive oxygen intermediate (ROI) production after activation with zymosan or phorbol myristate acetate (PMA). However, untreated oyster hemocytes also produce ROI and RNI (reactive nitrogen intermediates) after bleeding even if not stimulated by Zymosan or PMA. Extensive investigation of this parameter by flow cytometry showed that, in vitro, ROI/RNI production by untreated hemocytes maintained in seawater appeared to be independent of both bacterial burden in the serum and non-self particle phagocytosis. ROI/ RNI production in granulocytes was higher than in hyalinocytes and could be intensified when activated by zymosan but not by PMA. Both cell types...
Tipo: Text Palavras-chave: NO synthase; NADPH oxidase; Flow cytometry; Reactive nitrogen intermediate RNI; Reactive oxygen intermediate ROI; Hemocytes; Crassostrea gigas.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3057.pdf
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